Difference between revisions of "Part:BBa K4759203:Experience"
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===Applications of BBa_K4759203=== | ===Applications of BBa_K4759203=== | ||
Selection of suitable plasmid | Selection of suitable plasmid | ||
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| + | First, we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate. | ||
https://static.igem.wiki/teams/4759/wiki/2-1.png | https://static.igem.wiki/teams/4759/wiki/2-1.png | ||
Latest revision as of 01:29, 10 October 2023
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Applications of BBa_K4759203
Selection of suitable plasmid
First, we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate.
Fig2-1: Selection of suitable plasmid. WT represents E. coli O1 strain. The blue-filled triangle represents the biomass (OD600). The red hollow triangle represents the conversion rate (%). Values and triangles represent the means and standard deviations of biological triplicates.
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