Difference between revisions of "Part:BBa K4800054"

Latest revision as of 12:10, 11 October 2023

pTrc99a-davB-davA-GabT

We use the laboratory conserved plasmid pET22b-T7-davB-T7-davA as templates, the fragments of davB and davA were amplified by PCR with a consensus RBS and Ptrc respectively. Subsequently, the Ptrc-RBS-davB-Ptrc-RBS-davA fragment was obtained by overlap PCR, which was then ligated into the linearized vector pTrc99a-GabT by In-fusion cloning method to obtain plasmid pTtrc99a-davB-davA-GabT.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 5122
Illegal XbaI site found at 6489
Illegal PstI site found at 6501
Illegal NgoMIV site found at 4057
Illegal AgeI site found at 3464
Illegal AgeI site found at 3713
Illegal AgeI site found at 5535
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 6685
Illegal BsaI site found at 7753
Illegal SapI.rc site found at 767

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4800054 short</partinfo>
-davB-davA-GabT gene encodes for carboxylate reductase, alcohol dehydrogenase and transaminase. These enzymes can realize the metabolic pathway from lysine to glutarate semialdehyde, and the three enzymes form a synthetic module including 5-HV.
+We use the laboratory conserved plasmid pET22b-T7-davB-T7-davA as templates, the fragments of davB and davA were amplified by PCR with a consensus RBS and Ptrc respectively. Subsequently, the Ptrc-RBS-davB-Ptrc-RBS-davA fragment was obtained by overlap PCR, which was then ligated into the linearized vector pTrc99a-GabT by In-fusion cloning method to obtain plasmid pTtrc99a-davB-davA-GabT.
 <!-- Add more about the biology of this part here