Difference between revisions of "Part:BBa K211002:Design"

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Designed with PCR.
 
Designed with PCR.
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[[Image:primer design.jpg]]
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Primer Sequence
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P1 RI7-N F         ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC
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P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG
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P3 RI7-N R         GAGAAGAA ATACATGGGTTTGTGGAG
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P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG
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P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG
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P6 RI7-C F         GATCATT TACTGCTTGCGCAACC                                                             
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BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC 
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BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC                                                         
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We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.
 
We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Latest revision as of 22:09, 21 October 2009

RI7-odr10 chimeric GPCR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with PCR. Primer design.jpg Primer Sequence

P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC

P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG

P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG

P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG

P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG

P6 RI7-C F GATCATT TACTGCTTGCGCAACC


BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC

BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC


We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Source

Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)

Rat olfactory protein, clone I7 (GenBank: M64386.1)

References

Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330