Difference between revisions of "Part:BBa K4897015"
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With reference to part BBa_K4329007, another sgRNA-A2 (sequence AAAGTCGCAATTGTATGCAC) is designed by our team BS_United_China in 2022 to target the ATP synthase subunit C also used, so that when it is combined with CAS9, two parts of the ATP synthase is destroyed, guaranteeing the death of the powerhouse of the bacteria. | With reference to part BBa_K4329007, another sgRNA-A2 (sequence AAAGTCGCAATTGTATGCAC) is designed by our team BS_United_China in 2022 to target the ATP synthase subunit C also used, so that when it is combined with CAS9, two parts of the ATP synthase is destroyed, guaranteeing the death of the powerhouse of the bacteria. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
EL222 in our blue light suicide system will activate the expression of CAS9 and its sgRNA sequence (here sgRNA-A2). The gene editing process takes place after that, disrupting two of the most crucial parts of the bacterial cell’s metabolic pathways, energy and protein production, which eventually cause the death of bacteria. | EL222 in our blue light suicide system will activate the expression of CAS9 and its sgRNA sequence (here sgRNA-A2). The gene editing process takes place after that, disrupting two of the most crucial parts of the bacterial cell’s metabolic pathways, energy and protein production, which eventually cause the death of bacteria. | ||
Latest revision as of 15:42, 9 October 2023
sgRNA-A2
With reference to part BBa_K4329007, another sgRNA-A2 (sequence AAAGTCGCAATTGTATGCAC) is designed by our team BS_United_China in 2022 to target the ATP synthase subunit C also used, so that when it is combined with CAS9, two parts of the ATP synthase is destroyed, guaranteeing the death of the powerhouse of the bacteria.
Usage and Biology
EL222 in our blue light suicide system will activate the expression of CAS9 and its sgRNA sequence (here sgRNA-A2). The gene editing process takes place after that, disrupting two of the most crucial parts of the bacterial cell’s metabolic pathways, energy and protein production, which eventually cause the death of bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]