Difference between revisions of "Part:BBa K4788001"
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<partinfo>BBa_K4788001 short</partinfo> | <partinfo>BBa_K4788001 short</partinfo> | ||
− | codes FadR, it is a transcription factor that can bind to the FadBA promoter, inhibit initiation, and be released by oleoyl CoA metabolized from oleic acid. | + | codes FadR, it is a transcription factor that can bind to the FadBA promoter, inhibit initiation, and be released by oleoyl CoA metabolized from oleic acid. For specific information on oleic acid inducers, please refer to <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4788002"> BBa_K4788002</a ></html> |
− | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | It is a basic coding protein component that can combine with the FadO operator and FadBA promoter gene to form an oleic acid inducer to perform rich functions. | ||
+ | |||
+ | ===After forming composite components=== | ||
+ | The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects. | ||
+ | |||
+ | |||
+ | ===Reference=== | ||
+ | Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297. | ||
+ | |||
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Latest revision as of 01:32, 12 October 2023
FadR transcription factor
codes FadR, it is a transcription factor that can bind to the FadBA promoter, inhibit initiation, and be released by oleoyl CoA metabolized from oleic acid. For specific information on oleic acid inducers, please refer to BBa_K4788002
Usage and Biology
It is a basic coding protein component that can combine with the FadO operator and FadBA promoter gene to form an oleic acid inducer to perform rich functions.
After forming composite components
The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects.
Reference
Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]