Difference between revisions of "Part:BBa K4743033"

 
 
(3 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
 
This is the NMN production set for Kluryvermyces Marxianus
 
This is the NMN production set for Kluryvermyces Marxianus
 +
 +
<span class='h3bb'><big>'''Overall metabolic pathway of NMN'''</big></span>
 +
 +
NadA,NadB, NadE, NadV,prsA and, PnuC are involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.
 +
 +
<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nmn-metabolic-pathway.png" </html>
 +
 +
<center>Figure 1. The Matabolic pathway of NMN production.</center>
 +
 +
<span class='h3bb'><big>'''quantitative test of NMN'''</big></span>
 +
 +
<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nmn-hplc-igem-upload.png" </html>
 +
 +
<center>Figure 2. NMN HPLC data.</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:19, 10 October 2023


NMN production set

This is the NMN production set for Kluryvermyces Marxianus

Overall metabolic pathway of NMN

NadA,NadB, NadE, NadV,prsA and, PnuC are involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 1. The Matabolic pathway of NMN production.

quantitative test of NMN

Figure 2. NMN HPLC data.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1910
    Illegal EcoRI site found at 4397
    Illegal EcoRI site found at 6103
    Illegal EcoRI site found at 8446
    Illegal EcoRI site found at 10267
    Illegal EcoRI site found at 12682
    Illegal XbaI site found at 3266
    Illegal XbaI site found at 3485
    Illegal XbaI site found at 3731
    Illegal XbaI site found at 3828
    Illegal XbaI site found at 11405
    Illegal SpeI site found at 1715
    Illegal SpeI site found at 4202
    Illegal SpeI site found at 6096
    Illegal PstI site found at 3598
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1910
    Illegal EcoRI site found at 4397
    Illegal EcoRI site found at 6103
    Illegal EcoRI site found at 8446
    Illegal EcoRI site found at 10267
    Illegal EcoRI site found at 12682
    Illegal NheI site found at 5798
    Illegal NheI site found at 5874
    Illegal SpeI site found at 1715
    Illegal SpeI site found at 4202
    Illegal SpeI site found at 6096
    Illegal PstI site found at 3598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1910
    Illegal EcoRI site found at 4397
    Illegal EcoRI site found at 6103
    Illegal EcoRI site found at 8446
    Illegal EcoRI site found at 10267
    Illegal EcoRI site found at 12682
    Illegal BglII site found at 7272
    Illegal BglII site found at 11009
    Illegal BamHI site found at 642
    Illegal BamHI site found at 2580
    Illegal BamHI site found at 5067
    Illegal BamHI site found at 6773
    Illegal BamHI site found at 7124
    Illegal BamHI site found at 7732
    Illegal BamHI site found at 9116
    Illegal XhoI site found at 11603
    Illegal XhoI site found at 12329
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1910
    Illegal EcoRI site found at 4397
    Illegal EcoRI site found at 6103
    Illegal EcoRI site found at 8446
    Illegal EcoRI site found at 10267
    Illegal EcoRI site found at 12682
    Illegal XbaI site found at 3266
    Illegal XbaI site found at 3485
    Illegal XbaI site found at 3731
    Illegal XbaI site found at 3828
    Illegal XbaI site found at 11405
    Illegal SpeI site found at 1715
    Illegal SpeI site found at 4202
    Illegal SpeI site found at 6096
    Illegal PstI site found at 3598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1910
    Illegal EcoRI site found at 4397
    Illegal EcoRI site found at 6103
    Illegal EcoRI site found at 8446
    Illegal EcoRI site found at 10267
    Illegal EcoRI site found at 12682
    Illegal XbaI site found at 3266
    Illegal XbaI site found at 3485
    Illegal XbaI site found at 3731
    Illegal XbaI site found at 3828
    Illegal XbaI site found at 11405
    Illegal SpeI site found at 1715
    Illegal SpeI site found at 4202
    Illegal SpeI site found at 6096
    Illegal PstI site found at 3598
    Illegal AgeI site found at 1923
    Illegal AgeI site found at 4410
    Illegal AgeI site found at 5226
    Illegal AgeI site found at 6116
    Illegal AgeI site found at 8459
    Illegal AgeI site found at 10280
    Illegal AgeI site found at 12695
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3365