Difference between revisions of "Part:BBa K4585016"

 
 
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<partinfo>BBa_K4585016 short</partinfo>
 
<partinfo>BBa_K4585016 short</partinfo>
  
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The purpose of constructing this linear vector was to perform homologous recombination with the GnRH-C homologous recombination fragment (BBa_K4585000) to obtain the pcDNA3.1(+)-GnRH-C plasmid (BBa_K45850011).
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We based on the sequence of the pcDNA3.1(+)-GnRH-C plasmid. Special primers were designed to cut the plasmid into a linear sequence by PCR. The two ends of this linear sequence can be complementary to the GnRH-C homologous recombination fragment to obtain the target product, the pcDNA3.1(+)-GnRH-C plasmid (BBa_K4585011).
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<html>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<head>
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</head>
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<body>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                1 Diagrams
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                <p style="text-align: center;">
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/the-agarose-gel-lectrophoresis-of-pcdna3-1-gnrh-c-linearized-vector.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector</p>
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                2 Caution
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                <p>The two ends of the pcDNA3.1(+)-GnRH-C linearized vector need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.
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                </p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4585016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4585016 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4585016 parameters</partinfo>
 
<partinfo>BBa_K4585016 parameters</partinfo>
 
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Latest revision as of 03:06, 11 October 2023

pcDNA3.1(+)-GnRH-C linearized vector

We based on the sequence of the pcDNA3.1(+)-GnRH-C plasmid. Special primers were designed to cut the plasmid into a linear sequence by PCR. The two ends of this linear sequence can be complementary to the GnRH-C homologous recombination fragment to obtain the target product, the pcDNA3.1(+)-GnRH-C plasmid (BBa_K4585011).

1 Diagrams


Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector

2 Caution

The two ends of the pcDNA3.1(+)-GnRH-C linearized vector need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 249
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal XhoI site found at 901
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 249
    Illegal NgoMIV site found at 1341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882