Difference between revisions of "Part:BBa J36851:Experience"
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| + | The [http://2009.igem.org/Team:Washington 2009 University of Washington iGEM Team] tried to use this part to display strepavidin on the surface of the cell, and could not determine significant binding of cells expressing this part to biotin by either flow cytometry or microscopy. For details, see this part's main page or the 2009 UW iGEM wiki. | ||
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| − | After extensive testing of this part, we were not able to verify that binding was occurring between the streptavadin on the surface and a biotinylated flourophore we used for testing. We were able to see the protein on a protein gel, but we were not able to prove that it was correctly functioning. For more on our results, please see the [http://2009.igem.org/Team:Washington Washington iGEM 2009 page]. | + | |After extensive testing of this part, we were not able to verify that binding was occurring between the streptavadin on the surface and a biotinylated flourophore we used for testing. We were able to see the protein on a protein gel, but we were not able to prove that it was correctly functioning. For more on our results, please see the [http://2009.igem.org/Team:Washington Washington iGEM 2009 page]. |
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Latest revision as of 03:08, 22 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J36851
User Reviews
The [http://2009.igem.org/Team:Washington 2009 University of Washington iGEM Team] tried to use this part to display strepavidin on the surface of the cell, and could not determine significant binding of cells expressing this part to biotin by either flow cytometry or microscopy. For details, see this part's main page or the 2009 UW iGEM wiki.
UNIQ5ea2cfa290040842-partinfo-00000000-QINU UNIQ5ea2cfa290040842-partinfo-00000001-QINU