Difference between revisions of "Part:BBa K4600002:Experience"

 
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===Applications of BBa_K4600002===
 
===Applications of BBa_K4600002===
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        <img src="https://static.igem.wiki/teams/4600/wiki/tetr-data.png" alt="Team" style="width: 600px;" class="center">
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        <figcaption>Figure 1: Colony PCR where a pJBEI-6411 plasmid harbouring the TetR and AcrB pump sequence, were tested for uptake. Used a 1kb plus DNA ladder.</figcaption>
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We designed primers that bind on either side of the TetR sequence at a distance of 709 base pairs. Only plasmids with the insert should get be cloned in the PCR. The successful colony PCR suggests that there was a successful uptake of the part by the host.
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===User Reviews===
 
===User Reviews===

Latest revision as of 02:38, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4600002

Team
Figure 1: Colony PCR where a pJBEI-6411 plasmid harbouring the TetR and AcrB pump sequence, were tested for uptake. Used a 1kb plus DNA ladder.
We designed primers that bind on either side of the TetR sequence at a distance of 709 base pairs. Only plasmids with the insert should get be cloned in the PCR. The successful colony PCR suggests that there was a successful uptake of the part by the host.

User Reviews

UNIQ89453f7379415184-partinfo-00000001-QINU UNIQ89453f7379415184-partinfo-00000002-QINU