Difference between revisions of "Part:BBa K4593019"

Latest revision as of 21:07, 8 October 2023

Endolysin combinative expression circuit in E.coli

This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vivo. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract.

Detailed characterization of each endolysin is documented in their respective basic parts.

Please see the design page for usage instructions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2537
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2195
Illegal NgoMIV site found at 3021
Illegal NgoMIV site found at 3695
Illegal AgeI site found at 2064
Illegal AgeI site found at 3165
Illegal AgeI site found at 3393
Illegal AgeI site found at 3564
Illegal AgeI site found at 3677
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4593019 short</partinfo>
-This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vitro. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract.
+This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vivo. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract.
 Detailed characterization of each endolysin is documented in their respective basic parts.