Difference between revisions of "Part:BBa K4722008"
 
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The genetic construct was ligated into a pET28a plasmid vector and subsequently introduced into Escherichia coli strain BL21 (DE3).
 
The genetic construct was ligated into a pET28a plasmid vector and subsequently introduced into Escherichia coli strain BL21 (DE3).
 
NicX was genetically connected with SP to enable its direct translation onto the surface of BL21. This innovation eliminated the need for protein purification steps, allowing for the direct utilization of E. coli as a host for enzymes in various applications.
 
NicX was genetically connected with SP to enable its direct translation onto the surface of BL21. This innovation eliminated the need for protein purification steps, allowing for the direct utilization of E. coli as a host for enzymes in various applications.
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===Enzyme Activity===
 
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TBD
 
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===References===
 
===References===

Latest revision as of 17:33, 8 October 2023

3-Succinoyl-pyridine

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

3-Succinoyl-pyridine (SP) is a precursor compound with pivotal significance in the synthesis of depressant substances[1]. The biosynthesis of SP is intricately linked to the bioconversion of tobacco waste and is executed within the entirety of the cellular structure of the genetically engineered Pseudomonas strain S16dspm. SP represents a derivative of nicotine, characterized by its molecular simplicity as a pyridine compound. Remarkably, this compound possesses the capacity for transformation into a mammalian antihypertensive agent, namely ω-isopropyl-(propionyl)-L-proline[2].


Design Consideration

The genetic construct was ligated into a pET28a plasmid vector and subsequently introduced into Escherichia coli strain BL21 (DE3). NicX was genetically connected with SP to enable its direct translation onto the surface of BL21. This innovation eliminated the need for protein purification steps, allowing for the direct utilization of E. coli as a host for enzymes in various applications.

References

  1. ↑ Wang, S. N., Liu, Z., Tang, H. Z., Meng, J., & Xu, P. (2007). Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16. Microbiology, 153(5), 1556-1565. https://doi.org/10.1099/mic.0.2006/005223-0
  2. ↑ Wang, W., Xu, P., & Tang, H. (2015). Sustainable production of valuable compound 3-succinoyl-pyridine by genetically engineering Pseudomonas putida using the tobacco waste. Scientific Reports, 5(1), 16411. https://doi.org/10.1038/srep16411