Difference between revisions of "Part:BBa K4800043"

Latest revision as of 08:57, 11 October 2023

pTrc99a-SAV2585

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SAV2585.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 4486
Illegal PstI site found at 3174
Illegal PstI site found at 4498
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 3174
Illegal PstI site found at 4498
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4480
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 4486
Illegal PstI site found at 3174
Illegal PstI site found at 4498
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 4486
Illegal PstI site found at 3174
Illegal PstI site found at 4498
Illegal NgoMIV site found at 2710
Illegal NgoMIV site found at 3374
Illegal NgoMIV site found at 3403
Illegal NgoMIV site found at 3448
Illegal NgoMIV site found at 4252
Illegal NgoMIV site found at 4366
Illegal AgeI site found at 3059
Illegal AgeI site found at 3110
Illegal AgeI site found at 3185
Illegal AgeI site found at 3299
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4682
Illegal BsaI site found at 5750
Illegal SapI.rc site found at 767

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4800043 short</partinfo>
-Adenosylmethionine-8-amino-7-oxononanoate transaminase from <i>Streptomyces avermitilis</i>. A pyridoxal 5'-phosphate enzyme. S-adenosylhomocysteine can also act as donor.
+We linearized the pTrc99a vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-SAV2585.
 <!-- Add more about the biology of this part here