Difference between revisions of "Part:BBa K4800044"
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<partinfo>BBa_K4800044 short</partinfo> | <partinfo>BBa_K4800044 short</partinfo> | ||
− | + | We linearized the pTrc99a vector using restriction endonuclease and connected the target gene QLH to it through homologous recombination to obtain the recombinant plasmid pTrc99a-QLH. | |
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Latest revision as of 09:01, 11 October 2023
pTrc99a-QLH
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene QLH to it through homologous recombination to obtain the recombinant plasmid pTrc99a-QLH.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3988
Illegal PstI site found at 4000 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4000
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3982
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3988
Illegal PstI site found at 4000 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3988
Illegal PstI site found at 4000
Illegal NgoMIV site found at 3209
Illegal NgoMIV site found at 3758
Illegal AgeI site found at 3238 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4184
Illegal BsaI site found at 5252
Illegal SapI.rc site found at 767