Difference between revisions of "Part:BBa K4800046"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4800046 short</partinfo>
 
<partinfo>BBa_K4800046 short</partinfo>
  
−
4-aminobutyrate-2-oxoglutarate transaminase from <i>Escherichia coli K-12</i>. Requires pyridoxal phosphate. Some preparations also act on beta-alanine, 5-aminopentanoate and (R,S)-3-amino-2-methylpropanoate.
+
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-GabT.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:09, 11 October 2023


pTrc99a-GabT

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-GabT.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3889
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3850
    Illegal PstI site found at 3889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3850
    Illegal BamHI site found at 3871
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3889
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3889
    Illegal AgeI site found at 2897
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4073
    Illegal BsaI site found at 5141
    Illegal SapI.rc site found at 767