Difference between revisions of "Part:BBa K4800048"
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<partinfo>BBa_K4800048 short</partinfo> | <partinfo>BBa_K4800048 short</partinfo> | ||
− | + | We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-PatA. | |
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Latest revision as of 10:41, 11 October 2023
pTrc99a-YahK-PatA
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-PatA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3946
Illegal AgeI site found at 4453 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5331
Illegal BsaI site found at 6399
Illegal SapI.rc site found at 767