Difference between revisions of "Part:BBa K4665120:Design"

(→‎Design Notes)
(→‎Source)
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Coding sequence for the thermostable α-carbonic anhydrase SazCA which catalyzes the reversible hydration reaction of
 
−
 
  
 
This sequence will be attached to a flexible linker sequence at its 5' end for the final construct of our fusion protein
 
This sequence will be attached to a flexible linker sequence at its 5' end for the final construct of our fusion protein
Line 14: Line 12:
 
===Source===
 
===Source===
  
−
This part is derived from the genome of extremophilic bacterium <i>Sulfurihydrogenibium azorense</i>. Our sequence is derived from the paper by Zhu et al., (2022), which codon-optimized for the SazCA sequence for <i>E. coli BL21(DE3)</i>.
+
This part is derived from the genome of extremophilic bacterium <i>Sulfurihydrogenibium azorense</i>.  
 +
 
 +
Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for <i>E. coli BL21(DE3)</i>.
  
 
===References===
 
===References===
  
 
Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008
 
Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008

Latest revision as of 11:38, 10 October 2023


SazCA Carbonic Anhydrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence will be attached to a flexible linker sequence at its 5' end for the final construct of our fusion protein

Source

This part is derived from the genome of extremophilic bacterium Sulfurihydrogenibium azorense.

Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for E. coli BL21(DE3).

References

Zhu, Y., et.al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008