Difference between revisions of "Part:BBa K4800037"

 
 
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<partinfo>BBa_K4800037 short</partinfo>
 
<partinfo>BBa_K4800037 short</partinfo>
  
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Alcohol dehydrogenase from <i>Oenococcus oeni</i>.
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We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Adh1 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Adh1.
  
  

Latest revision as of 12:58, 12 October 2023


pTrc99a-Adh1

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Adh1 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Adh1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3350
    Illegal PstI site found at 3362
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3350
    Illegal PstI site found at 3362
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3350
    Illegal PstI site found at 3362
    Illegal AgeI site found at 2611
    Illegal AgeI site found at 2683
    Illegal AgeI site found at 3292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3546
    Illegal BsaI site found at 4614
    Illegal SapI.rc site found at 767