Difference between revisions of "Part:BBa K4800035"

Latest revision as of 06:39, 11 October 2023

pRSFDuet-MSMEG5739

We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MSMEG5739 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MSMEG5739.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1415
Illegal XbaI site found at 7230
Illegal PstI site found at 7242
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 7242
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 4705
Illegal BamHI site found at 3574
Illegal BamHI site found at 7224
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1415
Illegal XbaI site found at 7230
Illegal PstI site found at 7242
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1415
Illegal XbaI site found at 7230
Illegal PstI site found at 7242
Illegal NgoMIV site found at 3823
Illegal NgoMIV site found at 4418
Illegal NgoMIV site found at 4426
Illegal NgoMIV site found at 4838
Illegal NgoMIV site found at 6938
Illegal AgeI site found at 4565
Illegal AgeI site found at 4883
Illegal AgeI site found at 6121
Illegal AgeI site found at 7653
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 7474
Illegal BsaI site found at 7726
Illegal SapI site found at 660

Revision as of 12:35, 8 October 2023 (view source) Registry (Talk \| contribs)		Latest revision as of 06:39, 11 October 2023 (view source) ZQM 0907 (Talk \| contribs)
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	<partinfo>BBa_K4800035 short</partinfo>		<partinfo>BBa_K4800035 short</partinfo>

−	~~Carboxylic acid reductase from <i>Mycolicibacterium smegmatis MC2 155</i>.The enzyme contains an adenylation domain, a phosphopantetheinyl binding domain,~~ and ~~a reductase domain, and requires activation by attachment of a phosphopantetheinyl group. The enzyme activates its substrate to an adenylate form, followed by a transfer to~~ the ~~phosphopantetheinyl binding domain. The resulting thioester is subsequently transferred~~ to ~~the reductase domain, where~~ it ~~is reduced~~ to ~~an aldehyde and released~~.	+	We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MSMEG5739 to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MSMEG5739.