Difference between revisions of "Part:BBa K4800034"

 
 
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<partinfo>BBa_K4800034 short</partinfo>
 
<partinfo>BBa_K4800034 short</partinfo>
  
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Carboxylic acid reductase from <i>Mycobacterium avium</i>.The enzyme contains an adenylation domain, a phosphopantetheinyl binding domain, and a reductase domain, and requires activation by attachment of a phosphopantetheinyl group. The enzyme activates its substrate to an adenylate form, followed by a transfer to the phosphopantetheinyl binding domain. The resulting thioester is subsequently transferred to the reductase domain, where it is reduced to an aldehyde and released.
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We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MpCAR to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MpCAR.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:17, 11 October 2023


pRSFDuet-MpCAR

We linearized the pRSFDuet vector using restriction endonuclease and connected the target gene MpCAR to it through homologous recombination to obtain the recombinant plasmid pRSFDuet-MpCAR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7170
    Illegal PstI site found at 7182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4321
    Illegal BglII site found at 4609
    Illegal BglII site found at 5244
    Illegal BglII site found at 5530
    Illegal BamHI site found at 3574
    Illegal BamHI site found at 7164
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7170
    Illegal PstI site found at 7182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1415
    Illegal XbaI site found at 7170
    Illegal PstI site found at 7182
    Illegal NgoMIV site found at 4327
    Illegal NgoMIV site found at 4345
    Illegal NgoMIV site found at 4712
    Illegal NgoMIV site found at 4895
    Illegal AgeI site found at 3857
    Illegal AgeI site found at 4169
    Illegal AgeI site found at 4274
    Illegal AgeI site found at 5102
    Illegal AgeI site found at 5459
    Illegal AgeI site found at 5984
    Illegal AgeI site found at 6794
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7414
    Illegal BsaI site found at 7666
    Illegal SapI site found at 660