Difference between revisions of "Part:BBa K200017"

Latest revision as of 19:51, 18 October 2009

RBS + OtsB

Composite part containing RBS for ligation to OtsA and promoter for production of trehalose.

RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter, RBS and the first enzyme (OtsA) upstream of the RBS. Furthermore, to close the unit a double terminator would be required downstream of the OtsB gene.

OtsB ( BBa_K200006) is the gene coding for the enzyme trehalose phosphatase. Here it has been combined with an RBS (BBa_B0034).Both OtsA and OtsB are required for the production of trehalose, so this BioBrick should be used in combination with the OtsA gene (BBa_K200005).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 473
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Composite part containing RBS for ligation to OtsA and promoter for production of trehalose.
-RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter and the first enzyme (OtsA) upstream of the RBS. To close the unit a double terminator would be required downstream of the OtsB gene.
+RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter, RBS and the first enzyme (OtsA) upstream of the RBS. Furthermore, to close the unit a double terminator would be required downstream of the OtsB gene.
+OtsB ([[Part:BBa_K200006 | BBa_K200006]]) is the gene coding for the enzyme trehalose phosphatase. Here it has been combined with an RBS ([[Part:BBa_B0034 |BBa_B0034]]).Both OtsA and OtsB are required for the production of trehalose, so this BioBrick should be used in combination with the OtsA gene ([[Part:BBa_K200005 |BBa_K200005]]).