Difference between revisions of "Part:BBa K200017"

 
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Composite part containing RBS for ligation to OtsA and promoter for production of trehalose.
 
Composite part containing RBS for ligation to OtsA and promoter for production of trehalose.
  
RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter and the first enzyme (OtsA) upstream of the RBS. To close the unit a double terminator would be required downstream of the OtsB gene.  
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RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter, RBS and the first enzyme (OtsA) upstream of the RBS. Furthermore, to close the unit a double terminator would be required downstream of the OtsB gene.
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OtsB ([[Part:BBa_K200006 | BBa_K200006]]) is the gene coding for the enzyme trehalose phosphatase. Here it has been combined with an RBS ([[Part:BBa_B0034 |BBa_B0034]]).Both OtsA and OtsB are required for the production of trehalose, so this BioBrick should be used in combination with the OtsA gene ([[Part:BBa_K200005 |BBa_K200005]]).  
  
  

Latest revision as of 19:51, 18 October 2009

RBS + OtsB

Composite part containing RBS for ligation to OtsA and promoter for production of trehalose.


RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter, RBS and the first enzyme (OtsA) upstream of the RBS. Furthermore, to close the unit a double terminator would be required downstream of the OtsB gene.


OtsB ( BBa_K200006) is the gene coding for the enzyme trehalose phosphatase. Here it has been combined with an RBS (BBa_B0034).Both OtsA and OtsB are required for the production of trehalose, so this BioBrick should be used in combination with the OtsA gene (BBa_K200005).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]