Difference between revisions of "Part:BBa K190023:Design"

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===Source===
 
===Source===
  
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''E. coli'' TOP10
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The arsR promoter is present in most ''E. coli'' strains, and is regulated by ArsR dimer complex. Using the sequence of the promoter in our own ''E. coli'' TOP10 cells, oligo's were designed and annealed to produce the promoter with EcoRI and SpeI (prefix and suffix) overhangs.
  
 
===References===
 
===References===
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 +
1) C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal ''arsR'' Gene of ''Escherichia coli'' Encodes a ''trans''-acting Metalloregulatory Protein, ''The Journal of Biological Chemistry'', Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.

Latest revision as of 18:15, 21 October 2009

Arsenic Promoter (ArsR regulated) with own RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The arsR promoter is present in most E. coli strains, and is regulated by ArsR dimer complex. Using the sequence of the promoter in our own E. coli TOP10 cells, oligo's were designed and annealed to produce the promoter with EcoRI and SpeI (prefix and suffix) overhangs.

References

1) C. Xu, W. Shi, and B.P. Rosen (1996) The Chromosomal arsR Gene of Escherichia coli Encodes a trans-acting Metalloregulatory Protein, The Journal of Biological Chemistry, Vol. 271, No. 5, Issue of February 2, pp. 2427–2432.