Difference between revisions of "Part:BBa K4893001:Experience"
| (2 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| + | |||
| + | We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubate PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor. | ||
===Applications of BBa_K4893001=== | ===Applications of BBa_K4893001=== | ||
| + | |||
| + | Glucose sensors, enzyme fuel cells | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 05:44, 12 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
We tested to determine whether pyocyanin could be an electron acceptor of the PQQGDH and glucose oxidation reaction for the purpose of a pyocyanin biosensor. The protein was successfully expressed by Escherichia coli BL21 DE3 cells and tested with PQQGDH, although results were inconclusive and require further activity testing. One recommendation is to incubate PQQGDH with Ca2+ ions to improve the metal-ion coordinate binding between the GDH and PQQ cofactor.
Applications of BBa_K4893001
Glucose sensors, enzyme fuel cells
User Reviews
UNIQc04abeb8daf86f4a-partinfo-00000000-QINU UNIQc04abeb8daf86f4a-partinfo-00000001-QINU