Difference between revisions of "Part:BBa K4604037"

 
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<partinfo>BBa_K4604037 short</partinfo>
 
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MazE is the antitoxin.
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In this BioBrick we fused MazE together with a HA-tag using a GS-linker. This HA-tag can be used for protein detection via western blot. Also used and further characterized in BioBricks <a href="https://parts.igem.org/Part:BBa_K4604035">BBa_K4604035</a> and <a href="https://parts.igem.org/Part:BBa_K4604023">BBa_K4604023</a>.</html>
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===Usage and Biology===
 
===Usage and Biology===
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Toxin-antitoxin systems (TA-systems) play a crucial role in plasmid stability for naturally occurring plasmids[1]. Usually, the toxin targets essential cellular functions and causes growth arrest or cell death, to which the antitoxin acts as a counterpart. Toxin and antitoxin exhibit differences in their stability and lifespan[2]. While the antitoxin has a shortened lifespan due to its sensitivity to degradation, the toxin has a longer lifespan and is more stable. If the plasmid that contains the TA-system is lost, the antitoxin is rapidly degraded and the toxin concentration increases, leading to cell death. Therefore, when first discovered, TA systems were called “addiction modules” that ensure plasmid retention. The labile MazE (antitoxin) acts as a neutralizer to the stable MazF (toxin), which acts as an endoribonuclease. When MazF is present freely in the cell it cuts cellular RNA which ultimately leads to cell death.
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<TR><TH><img src="https://static.igem.wiki/teams/4604/wiki/description/hexamer-mazef.png" width="700" border="3">
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<span style="font-size: smaller"><b>Figure 1: 3D structure of MazE/MazF hexamer.</b> Pdb file was taken from the <a href="https://www.rcsb.org/structure/1ub4">Protein Data Bank,</a> display was done with <a href="molstar.org">Molstar</a>.</span></html>
  
 
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<partinfo>BBa_K4604037 parameters</partinfo>
 
<partinfo>BBa_K4604037 parameters</partinfo>
 
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===References===
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[1] Ni S, Li B, Tang K, Yao J, Wood TK, Wang P, et al. Conjugative plasmid-encoded toxin–antitoxin system PrpT/PrpA directly controls plasmid copy number. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2021 Jan 22;118(4). Available from: https://doi.org/10.1073/pnas.2011577118
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[2] Brzozowska I, Zielenkiewicz U. Regulation of toxin–antitoxin systems by proteolysis. Plasmid [Internet]. 2013 Jul 1;70(1):33–41. Available from: https://doi.org/10.1016/j.plasmid.2013.01.007

Latest revision as of 11:09, 11 October 2023


MazE

In this BioBrick we fused MazE together with a HA-tag using a GS-linker. This HA-tag can be used for protein detection via western blot. Also used and further characterized in BioBricks BBa_K4604035 and BBa_K4604023.


Usage and Biology

Toxin-antitoxin systems (TA-systems) play a crucial role in plasmid stability for naturally occurring plasmids[1]. Usually, the toxin targets essential cellular functions and causes growth arrest or cell death, to which the antitoxin acts as a counterpart. Toxin and antitoxin exhibit differences in their stability and lifespan[2]. While the antitoxin has a shortened lifespan due to its sensitivity to degradation, the toxin has a longer lifespan and is more stable. If the plasmid that contains the TA-system is lost, the antitoxin is rapidly degraded and the toxin concentration increases, leading to cell death. Therefore, when first discovered, TA systems were called “addiction modules” that ensure plasmid retention. The labile MazE (antitoxin) acts as a neutralizer to the stable MazF (toxin), which acts as an endoribonuclease. When MazF is present freely in the cell it cuts cellular RNA which ultimately leads to cell death.

Figure 1: 3D structure of MazE/MazF hexamer. Pdb file was taken from the Protein Data Bank, display was done with Molstar.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Ni S, Li B, Tang K, Yao J, Wood TK, Wang P, et al. Conjugative plasmid-encoded toxin–antitoxin system PrpT/PrpA directly controls plasmid copy number. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2021 Jan 22;118(4). Available from: https://doi.org/10.1073/pnas.2011577118

[2] Brzozowska I, Zielenkiewicz U. Regulation of toxin–antitoxin systems by proteolysis. Plasmid [Internet]. 2013 Jul 1;70(1):33–41. Available from: https://doi.org/10.1016/j.plasmid.2013.01.007