Difference between revisions of "Part:BBa K4604017"
 
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<partinfo>BBa_K4604017 short</partinfo>
 
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BioBrick piG_02a is a plasmid consisting of the tet promoter/repressor, the modified t7 RBS, a AdoCbl riboswitch, MazF fused togther with mTurquoise and the rrnB terminator. The backbone we used in the experiments is pGGAselect. This version on the plasmid is leaky, a modified version of it is uploaded as BioBrick BBa_K4604018.
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BioBrick piG_02a consits of the tet promoter/repressor, an AdoCbl riboswitch, <i>mazF</i> fused togther with mTurquoise and the rrnB terminator. The backbone we used in the experiments is pGGAselect. This version on the plasmid is leaky, a modified version of it is uploaded as BioBrick <a href="https://parts.igem.org/Part:BBa_K4604018">BBa_K4604018</a>.</html>
  
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===Usage and Biology===
 
  
 
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Latest revision as of 17:26, 11 October 2023


piG_02a (leaky_tetR_riboK12_mazF_mTurq)

BioBrick piG_02a consits of the tet promoter/repressor, an AdoCbl riboswitch, mazF fused togther with mTurquoise and the rrnB terminator. The backbone we used in the experiments is pGGAselect. This version on the plasmid is leaky, a modified version of it is uploaded as BioBrick BBa_K4604018.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2265