Difference between revisions of "Part:BBa K4759225:Design"

 
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===Design Notes===
 
===Design Notes===
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1
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Site-directed mutagenesis of the PetF protein
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===Source===
 
===Source===
  
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1
 
  
 
===References===
 
===References===
 +
[1] Li, S.; Du, L.; Bernhardt, R. Redox Partners: Function Modulators of Bacterial P450 Enzymes. Trends Microbiol 2020, 28, 445-454. DOI: 10.1016/j.tim.2020.02.012
 +
 +
[2] Lou, D.; Tan, J.; Zhu, L.; Ji, S.; Tang, S.; Yao, K.; Han, J.; Wang, B. Engineering Clostridium absonum 7alpha-hydroxysteroid Dehydrogenase for Enhancing Thermostability Based on Flexible Site and DeltaDeltaG Prediction. Protein Pept Lett 2018, 25, 230-235. DOI: 10.2174/0929866524666171113113100
 +
 +
[3] Sagadin, T.; Riehm, J.; Putkaradze, N.; Hutter, M. C.; Bernhardt, R. Novel approach to improve progesterone hydroxylation selectivity by CYP106A2 via rational design of adrenodoxin binding. FEBS J 2019, 286, 1240-1249. DOI: 10.1111/febs.14722
 +
 +
[4] Meng, S.; Li, Z.; Ji, Y.; Ruff, A. J.; Liu, L.; Davari, M. D.; Schwaneberg, U. Introduction of aromatic amino acids in electron transfer pathways yielded improved catalytic performance of cytochrome P450s. Chinese Journal of Catalysis 2023, 49, 81-90. DOI: 10.1016/s1872-2067(23)64445-6
 +
 +
[5] Tovchigrechko, A.; Vakser, I. A. GRAMM-X public web server for protein-protein docking. Nucleic Acids Res 2006, 34, W310-314. DOI: 10.1093/nar/gkl206

Latest revision as of 05:54, 12 October 2023


T7-RBS1-petH-RBS2-petF(D21W)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1336
    Illegal NotI site found at 1109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1336
    Illegal BglII site found at 1647
    Illegal BamHI site found at 1330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1336
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutagenesis of the PetF protein


Source

References

[1] Li, S.; Du, L.; Bernhardt, R. Redox Partners: Function Modulators of Bacterial P450 Enzymes. Trends Microbiol 2020, 28, 445-454. DOI: 10.1016/j.tim.2020.02.012

[2] Lou, D.; Tan, J.; Zhu, L.; Ji, S.; Tang, S.; Yao, K.; Han, J.; Wang, B. Engineering Clostridium absonum 7alpha-hydroxysteroid Dehydrogenase for Enhancing Thermostability Based on Flexible Site and DeltaDeltaG Prediction. Protein Pept Lett 2018, 25, 230-235. DOI: 10.2174/0929866524666171113113100

[3] Sagadin, T.; Riehm, J.; Putkaradze, N.; Hutter, M. C.; Bernhardt, R. Novel approach to improve progesterone hydroxylation selectivity by CYP106A2 via rational design of adrenodoxin binding. FEBS J 2019, 286, 1240-1249. DOI: 10.1111/febs.14722

[4] Meng, S.; Li, Z.; Ji, Y.; Ruff, A. J.; Liu, L.; Davari, M. D.; Schwaneberg, U. Introduction of aromatic amino acids in electron transfer pathways yielded improved catalytic performance of cytochrome P450s. Chinese Journal of Catalysis 2023, 49, 81-90. DOI: 10.1016/s1872-2067(23)64445-6

[5] Tovchigrechko, A.; Vakser, I. A. GRAMM-X public web server for protein-protein docking. Nucleic Acids Res 2006, 34, W310-314. DOI: 10.1093/nar/gkl206