Difference between revisions of "Part:BBa K4759208"
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| − | + | NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.<br> | |
| + | We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced. | ||
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| + | https://static.igem.wiki/teams/4759/wiki/protein-tags.png | ||
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| + | Fig1: The application of protein tags | ||
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Latest revision as of 08:41, 12 October 2023
T7-RBS4-Nus-linker-Olep
NusA protein (N-utilization substance A) is a class of transcription elongation antiterminator in E. coli with a molecular weight of about 55 kDa.
We fused six protein tags (SUMO, GST, MBP, TF, Trx, Nus) at the N-terminal of the Olep. Protein tags MBP and TF can significantly improve the solubility of Olep, but the catalytic efficiency of Olep was reduced.
Fig1: The application of protein tags
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1463
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1623
Illegal AgeI site found at 1721 - 1000COMPATIBLE WITH RFC[1000]