Difference between revisions of "Part:BBa K4759205:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Olep is selected to synthesize the above gene by adding histidine tags at the | + | Olep is selected to synthesize the above gene by adding histidine tags at the N-terminal |
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===Source=== | ===Source=== | ||
− | + | The solubilization-promoting tag MBP was from Group: iGEM10_Calgary. | |
===References=== | ===References=== |
Latest revision as of 13:59, 12 October 2023
T7-RBS4-MBP-linker-OleP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 446
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1229
Illegal AgeI site found at 1327 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 144
Design Notes
Olep is selected to synthesize the above gene by adding histidine tags at the N-terminal
Source
The solubilization-promoting tag MBP was from Group: iGEM10_Calgary.