Difference between revisions of "Part:BBa K4759203:Experience"

Latest revision as of 01:29, 10 October 2023

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4759203

Selection of suitable plasmid

First, we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate.

Fig2-1: Selection of suitable plasmid. WT represents E. coli O1 strain. The blue-filled triangle represents the biomass (OD600). The red hollow triangle represents the conversion rate (%). Values and triangles represent the means and standard deviations of biological triplicates.

User Reviews

UNIQ6037dbedc4cf51e1-partinfo-00000000-QINU UNIQ6037dbedc4cf51e1-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K4759203===
+Selection of suitable plasmid
+First, we selected high-copy plasmids pRSFDuet, medium-high copy plasmids pETDuet and pET28a, and low-copy plasmid pACYCDuet as carriers to express Olep in E. coli, and the results showed that the recombinant strains constructed with high-copy plasmid pRSFDuet as the carrier had the highest expression of p450 enzyme, cell growth rate, and substrate conversion rate.
+https://static.igem.wiki/teams/4759/wiki/2-1.png
+Fig2-1: Selection of suitable plasmid. WT represents E. coli O1 strain.
+The blue-filled triangle represents the biomass (OD600). The red hollow triangle represents the conversion rate (%). Values and triangles represent the means and standard deviations of biological triplicates.
 ===User Reviews===