Difference between revisions of "Part:BBa K4759035:Experience"

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===Applications of BBa_K4759035===
 
===Applications of BBa_K4759035===
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we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
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we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags  
  
 +
(SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of
 +
 +
Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
  
 
https://static.igem.wiki/teams/4759/wiki/protein-tags.png
 
https://static.igem.wiki/teams/4759/wiki/protein-tags.png
  
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  The application of protein tags
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The application of protein tags
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 16:10, 7 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4759035

we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags

(SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of

Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.

protein-tags.png

The application of protein tags

User Reviews

UNIQ6ece2b91dc4bcd1f-partinfo-00000000-QINU UNIQ6ece2b91dc4bcd1f-partinfo-00000001-QINU