Difference between revisions of "Part:BBa K4759002:Design"

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===References===
 
===References===
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[1]B H, H Y, J Z, et al. Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis[J]. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023, 10(6): e2205580.
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[2] B N, L R-S. Redox metabolism for improving whole-cell P450-catalysed terpenoid biosynthesis[J]. Critical reviews in biotechnology, 2021: 1-25.

Latest revision as of 14:35, 7 October 2023


camA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 37
    Illegal AgeI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this sequence has been codon-optimized for expression in Escherichia coli.


Source

a gene coding for protein PdR(CamA), CamA is a putidaredoxin reductase, this is a containing putidaredoxin (Pdx), the electron donor to cytochrome P450cam in Pseudomonas putida

References

[1]B H, H Y, J Z, et al. Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis[J]. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023, 10(6): e2205580.

[2] B N, L R-S. Redox metabolism for improving whole-cell P450-catalysed terpenoid biosynthesis[J]. Critical reviews in biotechnology, 2021: 1-25.