Difference between revisions of "Part:BBa K4759001:Design"

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===Design Notes===
 
===Design Notes===
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Co-expressed with other components，and Olep is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization and subcloning the above genes onto the plasmid pET-28a to obtain the recombinant plasmid pET-28a-oleP.
+
Co-expressed with other components，and Olep is selected to synthesize the above gene by adding histidine tags at the C-terminus through <i>E. coli</i> codon optimization and subcloning the above genes onto the plasmid pET-28a to obtain the recombinant plasmid pET-28a-oleP.
  
 
===Source===
 
===Source===
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a gene coding for multifunctional bacterial P450 monooxygenase CYP107D1(Olep)
+
P450 enzyme CYP107D1 derived from <i>Streptomyces</i>, abbreviated as Olep
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+
  
 
===References===
 
===References===
−
[1] G P, I F, C E, et al. Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate[J]. Biomolecules, 2020, 10(10).
+
[1] G P, I F, C E, et al. Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate[J]. Biomolecules, 2020, 10(10).<br>
 
[2] B H, H Y, J Z, et al. Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis[J]. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023, 10(6): e2205580
 
[2] B H, H Y, J Z, et al. Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis[J]. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023, 10(6): e2205580

Latest revision as of 06:43, 12 October 2023


OleP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 16
    Illegal AgeI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Co-expressed with other components，and Olep is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization and subcloning the above genes onto the plasmid pET-28a to obtain the recombinant plasmid pET-28a-oleP.

Source

P450 enzyme CYP107D1 derived from Streptomyces, abbreviated as Olep

References

[1] G P, I F, C E, et al. Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate[J]. Biomolecules, 2020, 10(10).
[2] B H, H Y, J Z, et al. Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis[J]. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023, 10(6): e2205580