Difference between revisions of "Part:BBa K4767012:Design"
(→References) |
Airplan mod (Talk | contribs) (→Source) |
||
| (2 intermediate revisions by one other user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | We fused the arsenic responding biosensor and the LuxR-based amplifier, using <i>mtrC</i> as a target gene to detect arsenic in the chassis of <i>Shewanella oneidensis</i> in a electroactive biofilm-based sensor (EAB-sensor) | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | Shewanella | + | This part is from <i>Escherichia coli</i>, <i>Vibrio fischeri</i> and <i>Shewanella oneidensis</i>,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015,BBa_R0062, BBa_K3102020 and BBa_K4767000. |
===References=== | ===References=== | ||
Latest revision as of 08:27, 8 October 2023
Pars-RBS-arsR-RBS-luxR(△2-162)-TT-PluxⅠ-RBS-luxR(△2-162)-RBS-mtrC -TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1543
Illegal BsaI.rc site found at 929
Design Notes
We fused the arsenic responding biosensor and the LuxR-based amplifier, using mtrC as a target gene to detect arsenic in the chassis of Shewanella oneidensis in a electroactive biofilm-based sensor (EAB-sensor)
Source
This part is from Escherichia coli, Vibrio fischeri and Shewanella oneidensis,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015,BBa_R0062, BBa_K3102020 and BBa_K4767000.
References
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.