Difference between revisions of "Part:BBa K4767009"
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4767009 short</partinfo>
 
<partinfo>BBa_K4767009 short</partinfo>
  
−
Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801),  <i>luxR</i>(△2-162)(BBa_K4767000), <i>cymA</i>(BBa_K2706008)and TT(BBa_B015). This composite part is a BioBrick.
+
Uses a factor P<sub><i>luxⅠ</i></sub>(BBa_R0062) ,strong RBS(BBa_J34801),  <i>luxR</i>(△2-162)(BBa_K4767000), <i>cymA</i>(BBa_K2706008)and TT(BBa_B0015). This composite part is a BioBrick.
  
 
===Usage and Biology===
 
===Usage and Biology===
−
This is the response part of the <i>luxR</i> amplifier with the <i>cymA</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>cymA</i> and <i>luxR</i>(△2-162). CymA will increase the ability for the <i>Shewanella oneidensis</i> to transfer the electrons, and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.
+
This is the response part of the <i>luxR</i> amplifier with the <i>cymA</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>cymA</i> and <i>luxR</i>(△2-162). CymA will increase the ability for the <i>Shewanella oneidensis</i> to transfer the electrons, and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the P<sub><i>luxⅠ</i></sub> promoter and activate its own transcription.
  
−
<center>[[File:BBa_K4767009.jpg]]</center>
+
<center>https://static.igem.wiki/teams/4767/wiki/part/img-1172.png</center>
  
 
<!-- -->
 
<!-- -->

Latest revision as of 08:07, 8 October 2023


PluxⅠ-RBS-luxR(△2-162)-RBS-cymA-TT

Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000), cymA(BBa_K2706008)and TT(BBa_B0015). This composite part is a BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the cymA gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express cymA and luxR(△2-162). CymA will increase the ability for the Shewanella oneidensis to transfer the electrons, and luxR(△2-162) will functions in a positive feedback loop as it can bind to the PluxⅠ promoter and activate its own transcription.

img-1172.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.