Difference between revisions of "Part:BBa K4767008"
 
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<partinfo>BBa_K4767008 short</partinfo>
 
<partinfo>BBa_K4767008 short</partinfo>
  
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Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), luxR(△2-162)(BBa_K4767000) and TT(BBa_B015).  
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Uses a factor P<sub><i>ars</i></sub>(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), luxR(△2-162)(BBa_K4767000) and TT(BBa_B0015). This part is an easy BioBrick.
  
 
===Usage and Biology===
 
===Usage and Biology===
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After the modification of the LuxR(Δ2-162), we applied the amplifier into the arsenic-response system we build. The transcriptional activator, LuxR(Δ2-162) was regulated by the arsR-Pars circuit, while reporter gene together with a second LuxR(Δ2-162) was placed under the promoter PluxI, which was activated by LuxR(Δ2-162). When arsenic is present, it activates the expression of LuxR(Δ2-162) in the first circuit, which turns on the expression of reporter and LuxR(Δ2-162) (Part 5)from the following circuit.
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After the modification of the LuxR(Δ2-162), we applied the amplifier into the arsenic-response system we build. The transcriptional activator, LuxR(Δ2-162) was regulated by the <i>arsR</i>-P<sub><i>ars</i></sub> circuit, while reporter gene together with a second LuxR(Δ2-162) was placed under the promoter P<sub><i>luxI</i></sub>, which was activated by LuxR(Δ2-162). When arsenic is present, it activates the expression of LuxR(Δ2-162) in the first circuit, which turns on the expression of reporter and LuxR(Δ2-162) (Part 5)from the following circuit.
  
 
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===References===
 
===References===
 
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.
 
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.
 +
 
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.
 
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.
  

Latest revision as of 08:07, 8 October 2023


Pars-RBS-arsR-RBS-luxR(△2-162)-TT

Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), luxR(△2-162)(BBa_K4767000) and TT(BBa_B0015). This part is an easy BioBrick.

Usage and Biology

After the modification of the LuxR(Δ2-162), we applied the amplifier into the arsenic-response system we build. The transcriptional activator, LuxR(Δ2-162) was regulated by the arsR-Pars circuit, while reporter gene together with a second LuxR(Δ2-162) was placed under the promoter PluxI, which was activated by LuxR(Δ2-162). When arsenic is present, it activates the expression of LuxR(Δ2-162) in the first circuit, which turns on the expression of reporter and LuxR(Δ2-162) (Part 5)from the following circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.