Difference between revisions of "Part:BBa K4767005"
 
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<partinfo>BBa_K4767005 short</partinfo>
 
<partinfo>BBa_K4767005 short</partinfo>
  
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Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), <i>luxR</i>(△2-162)(BBa_K4767000) and <i>gfp</i> coding region(BBa_E0040)and TT(BBa_B015). This part is an easy BioBrick.
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Uses a factor P<sub><i>luxⅠ</i></sub>(BBa_R0062) ,strong RBS(BBa_J34801), <i>luxR</i>(△2-162)(BBa_K4767000) and <i>gfp</i> coding region(BBa_E0040)and TT(BBa_B0015). This part is an easy BioBrick.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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This is the response part of the <i>luxR</i> amplifier with the <i>gfp</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>gfp</i> and <i>luxR</i>(△2-162), and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.
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This is the response part of the <i>luxR</i> amplifier with the <i>gfp</i> gene as the reporter. In our design, if <i>luxR</i>(△2-162) was expressed as a result of responding, this part can be activated to express <i>gfp</i> and <i>luxR</i>(△2-162), and <i>luxR</i>(△2-162) will functions in a positive feedback loop as it can bind to the P<i><sub>luxI</sub></i> promoter and activate its own transcription.
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[[File:BBa_K4767005.jpg]]
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<center>https://static.igem.wiki/teams/4767/wiki/part/img-1167.png</center>
  
 
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Latest revision as of 08:06, 8 October 2023


PluxⅠ-RBS-luxR(△2-162)-RBS-gfp-TT

Uses a factor PluxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000) and gfp coding region(BBa_E0040)and TT(BBa_B0015). This part is an easy BioBrick.


Usage and Biology

This is the response part of the luxR amplifier with the gfp gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express gfp and luxR(△2-162), and luxR(△2-162) will functions in a positive feedback loop as it can bind to the PluxI promoter and activate its own transcription.

img-1167.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1021

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.