Difference between revisions of "Part:BBa M45400"
 
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EL222-binding clone 1–20 bp (C120)
 
EL222-binding clone 1–20 bp (C120)
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<br><b>BS-united China 2023</b>: [[File:https://static.igem.wiki/teams/4897/wiki/safety/figure-8-cleaning-and-rinsing-lab-equipment.jpg|700px|thumb|center|Fg.1 pathway of increasing dethiobiotin synthase and biotin synthase]]
 
  
 
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<partinfo>BBa_M45400 parameters</partinfo>
 
<partinfo>BBa_M45400 parameters</partinfo>
 
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==Applications of BBa_M45400 by BS_United_China 2023==
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BS_united China 2023: we will use this binding region of EL222 to synthesize a blue light suicide system that will start when our engineering bacteria lost in the external environment is not exposed to blue light. Because our engineering E. coli will be killed during the absence of blue light, namely in the dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus biding to the EL222 binding region located in between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter. Therefore we used this part for EL222 to start CAS9 suicide system to ensure the safety of the environment.
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The detailed design of our team can be seen below:
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<html>
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<img src="https://static.igem.wiki/teams/4897/wiki/safety/fig-10-off-state-blue-light-suircide-system.png" width="500" height="auto">/
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</html>
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<html>
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<img src="https://static.igem.wiki/teams/4897/wiki/safety/fig-11-on-state-blue-light-suicide-system.png" width="500" height="auto">/
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</html>

Latest revision as of 01:56, 10 October 2023

Optogenetic Promoter C120

EL222-binding clone 1–20 bp (C120)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Applications of BBa_M45400 by BS_United_China 2023

BS_united China 2023: we will use this binding region of EL222 to synthesize a blue light suicide system that will start when our engineering bacteria lost in the external environment is not exposed to blue light. Because our engineering E. coli will be killed during the absence of blue light, namely in the dark or at night, we equipped our engineering bacteria with a special part to produce EL222, a light-regulated DNA-binding protein. This protein can harness blue light to drive the reorientation of its Light-Oxygen-Voltage (LOV) sensory and Helix-Turn-Helix (HTH) effector domains, resulting in its self-dimerization and thus biding to the EL222 binding region located in between −35 (TTGACA) and −10 (TATAAT) region of the luxI promoter. Therefore we used this part for EL222 to start CAS9 suicide system to ensure the safety of the environment. The detailed design of our team can be seen below:

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