Difference between revisions of "Part:BBa K4767004"
 
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<partinfo>BBa_K4767004 short</partinfo>
 
<partinfo>BBa_K4767004 short</partinfo>
  
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Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), <i>gfp</i> coding region(BBa_E0040)and double terminator TT(BBa_B015). This part is an easy BioBrick.
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Uses a factor P<i><sub>ars</sub></i>(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), <i>gfp</i> coding region(BBa_E0040)and double terminator TT(BBa_B0015). This part is an easy BioBrick.
  
 
===Usage and Biology===
 
===Usage and Biology===
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System comes from <i>Escherichia coli</i> genome, to eliminate this impact, we used another model bacteria <i>Shewanella oneidensis</i> as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity.
 
System comes from <i>Escherichia coli</i> genome, to eliminate this impact, we used another model bacteria <i>Shewanella oneidensis</i> as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity.
  
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[[File:BBa_K4767004.jpg]]
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<center>https://static.igem.wiki/teams/4767/wiki/part/img-1166.png</center>
  
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Fig. Fluorescence curve of the reporter strain with different arsenic concentrations
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<center>Fig 1. Fluorescence curve of the reporter strain with different arsenic concentrations.</center>
  
 
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Latest revision as of 08:05, 8 October 2023


Pars-RBS-arsR-RBS-gfP-TT

Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), gfp coding region(BBa_E0040)and double terminator TT(BBa_B0015). This part is an easy BioBrick.

Usage and Biology

This part is a basic biosensor for arsenic detection using gfp as the reporter gene. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the downstream genes. Since this ars System comes from Escherichia coli genome, to eliminate this impact, we used another model bacteria Shewanella oneidensis as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity.

img-1166.png
Fig 1. Fluorescence curve of the reporter strain with different arsenic concentrations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1160