Difference between revisions of "Part:BBa K4800011"

Latest revision as of 09:03, 11 October 2023

YcjQ Donor

YcjQ Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.

Document

Build:

Fig1.YcjQ Donor

The genes at both end of the YcjQ gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the YcjQ Donor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 631
Illegal AgeI site found at 1313
Illegal AgeI site found at 1321
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1373

@@ Line 20: / Line 20: @@
 <body>
-     <p style="font-size: 180%; font-weight: bold;">Build:
+     <p style="font-size: 150%; font-weight: bold;">Build:
-     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/acka-pta-donor.png" width="80%" height="80%"></p>
+     <p><center><img src="https://static.igem.wiki/teams/4800/wiki/parts/donor.png" width="40%" height="40%"></center></p>
      <div align="center">
-         <strong>Fig1.The genes at either end of the YcjQ gene were selected as the upper and lower homology arms</strong>
+         <strong>Fig1.YcjQ Donor</strong>
      </div>
-     <p>The genes at either end of the YcjQ gene were selected as the upper and lower homology arms and joined by overlap, the upper and lower homology arms were amplified separately, purified and recovered to obtain the ycjQ Donor.
+     <p>The genes at both end of the YcjQ gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the YcjQ Donor.
      </p >