Difference between revisions of "Part:BBa K4800010"

 
 
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<partinfo>BBa_K4800010 short</partinfo>
 
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ackA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
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AckA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
  
 
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    <title>Document</title>
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    <p style="font-size: 150%; font-weight: bold;">Build:
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    <p><center><img src="https://static.igem.wiki/teams/4800/wiki/parts/donor.png" width="60%" height="60%"></center></p>
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    <div align="center">
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        <strong>Fig1.ackA-pta Donor</strong>
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    </div>
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    <p>The genes at both end of the ackA-pta gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the ackA-pta Donor.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4800010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4800010 SequenceAndFeatures</partinfo>

Latest revision as of 05:25, 11 October 2023


ackA-pta Donor

AckA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.


Document

Build:

Fig1.ackA-pta Donor

The genes at both end of the ackA-pta gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the ackA-pta Donor.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 969
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 969
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 969
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]