Difference between revisions of "Part:BBa K4800004"

 
 
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<partinfo>BBa_K4800004 short</partinfo>
 
<partinfo>BBa_K4800004 short</partinfo>
  
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MmCAR is a carboxylic acid reductase. To improve the catalytic activity of 5-hydroxyvaleric acid, we have decided to perform site-specific mutations at the Q302 site of MmCAR, mutate glutamine to glutamate, and validate the changes in catalytic activity through whole cell catalysis.  
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MmCAR is a carboxylate reductase. In order to improve the catalytic activity of 5-hydroxyvaleric acid, we decided to mutate the 302nd amino acid in MmCAR to convert glutamine (Q) into glutamic acid (E). By changing the population of the residuals, we evaluated the effect of the mutations on MmCAR catalytic activity.
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<!-- Add more about the biology of this part here

Latest revision as of 05:09, 11 October 2023


MmCAR(Q302E)

MmCAR is a carboxylate reductase. In order to improve the catalytic activity of 5-hydroxyvaleric acid, we decided to mutate the 302nd amino acid in MmCAR to convert glutamine (Q) into glutamic acid (E). By changing the population of the residuals, we evaluated the effect of the mutations on MmCAR catalytic activity.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 427
    Illegal PstI site found at 454
    Illegal PstI site found at 1723
    Illegal PstI site found at 1750
    Illegal PstI site found at 2143
    Illegal PstI site found at 2639
    Illegal PstI site found at 3283
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 427
    Illegal PstI site found at 454
    Illegal PstI site found at 1723
    Illegal PstI site found at 1750
    Illegal PstI site found at 2143
    Illegal PstI site found at 2639
    Illegal PstI site found at 3283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 981
    Illegal BglII site found at 1607
    Illegal BglII site found at 1883
    Illegal BglII site found at 2574
    Illegal XhoI site found at 3523
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 427
    Illegal PstI site found at 454
    Illegal PstI site found at 1723
    Illegal PstI site found at 1750
    Illegal PstI site found at 2143
    Illegal PstI site found at 2639
    Illegal PstI site found at 3283
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 427
    Illegal PstI site found at 454
    Illegal PstI site found at 1723
    Illegal PstI site found at 1750
    Illegal PstI site found at 2143
    Illegal PstI site found at 2639
    Illegal PstI site found at 3283
    Illegal NgoMIV site found at 2251
    Illegal NgoMIV site found at 2313
    Illegal NgoMIV site found at 2530
    Illegal NgoMIV site found at 2977
    Illegal AgeI site found at 157
    Illegal AgeI site found at 1168
    Illegal AgeI site found at 1465
    Illegal AgeI site found at 2350
    Illegal AgeI site found at 2359
    Illegal AgeI site found at 3034
  • 1000
    COMPATIBLE WITH RFC[1000]