Difference between revisions of "Part:BBa K4621073"
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This part contains the spacer sequence used to target a specific DNA fragment of a 4-aminobutyrate--2-oxoglutarate transaminase gene, gabT in SCUT-3. It must be co-expressed with a sgRNA that has a 20-base pair complementary sequence to the target gene that dCas9 can effectively silence the gene of interest [1]. In our case, we used it to target the binding site of RNP to effectively repress the transcription of the target gene. As a result, the production of the target product is intricately regulated. | This part contains the spacer sequence used to target a specific DNA fragment of a 4-aminobutyrate--2-oxoglutarate transaminase gene, gabT in SCUT-3. It must be co-expressed with a sgRNA that has a 20-base pair complementary sequence to the target gene that dCas9 can effectively silence the gene of interest [1]. In our case, we used it to target the binding site of RNP to effectively repress the transcription of the target gene. As a result, the production of the target product is intricately regulated. | ||
| − | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | See BBa_K4621083 for details | ||
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Latest revision as of 11:08, 10 October 2023
Spacer of 441-sgRNA to target gabT
This part contains the spacer sequence used to target a specific DNA fragment of a 4-aminobutyrate--2-oxoglutarate transaminase gene, gabT in SCUT-3. It must be co-expressed with a sgRNA that has a 20-base pair complementary sequence to the target gene that dCas9 can effectively silence the gene of interest [1]. In our case, we used it to target the binding site of RNP to effectively repress the transcription of the target gene. As a result, the production of the target product is intricately regulated.
Usage and Biology
See BBa_K4621083 for details
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]