Difference between revisions of "Part:BBa K260015:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K260015 short</partinfo>
 
<partinfo>BBa_K260015 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
A strong promoter was necessary and we use [[Part:BBa_J23100|]]. An appropriate reading frame for the FRT site had to be found to prevent premature Stop-codons and hydrophobic amino acid residues. Further, the gene encoding TmpR was codon-optimised.
+
A strong promoter was necessary and we use [[Part:BBa_J23100|BBa_J23100]]. An appropriate reading frame for the FRT site had to be found to prevent premature Stop-codons and hydrophobic amino acid residues. Further, the gene encoding TmpR was codon-optimised.
  
  
Line 15: Line 14:
 
This part was synthesised by "Mr Gene" (Geneart) and is available in the following plasmid backbones:
 
This part was synthesised by "Mr Gene" (Geneart) and is available in the following plasmid backbones:
  
−
[[Part:BBa_K260004|]]: pMA-@CC2FosMB-00500bp
+
[[Part:BBa_K260004|BBa_K260004]]: pMA-@CC2FosMB-00500bp
  
−
[[Part:BBa_K260005|]]: pMA-@CC2FosMB-01000bp
+
[[Part:BBa_K260005|BBa_K260005]]: pMA-@CC2FosMB-01000bp
  
−
[[Part:BBa_K260006|]]: pMA-@CC2FosMB-02000bp
+
[[Part:BBa_K260006|BBa_K260006]]: pMA-@CC2FosMB-02000bp
  
−
[[Part:BBa_K260007|]]: pMA-@CC2FosMB-05000bp
+
[[Part:BBa_K260007|BBa_K260007]]: pMA-@CC2FosMB-05000bp
  
−
[[Part:BBa_K260008|]]: pMA-RQ-@CC2FosMB-10000bp
+
[[Part:BBa_K260008|BBa_K260008]]: pMA-RQ-@CC2FosMB-10000bp
  
 
===References===
 
===References===

Latest revision as of 15:29, 18 October 2009

P_FRT_dhfr. (strong promoter, translated FRT site, trimethoprim resistance)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong promoter was necessary and we use BBa_J23100. An appropriate reading frame for the FRT site had to be found to prevent premature Stop-codons and hydrophobic amino acid residues. Further, the gene encoding TmpR was codon-optimised.


Source

This part was synthesised by "Mr Gene" (Geneart) and is available in the following plasmid backbones:

BBa_K260004: pMA-@CC2FosMB-00500bp

BBa_K260005: pMA-@CC2FosMB-01000bp

BBa_K260006: pMA-@CC2FosMB-02000bp

BBa_K260007: pMA-@CC2FosMB-05000bp

BBa_K260008: pMA-RQ-@CC2FosMB-10000bp

References