Difference between revisions of "Part:BBa K4767012:Design"

Latest revision as of 08:27, 8 October 2023

Pars-RBS-arsR-RBS-luxR(△2-162)-TT-PluxⅠ-RBS-luxR(△2-162)-RBS-mtrC -TT

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 270
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1543
Illegal BsaI.rc site found at 929

Design Notes

We fused the arsenic responding biosensor and the LuxR-based amplifier, using mtrC as a target gene to detect arsenic in the chassis of Shewanella oneidensis in a electroactive biofilm-based sensor (EAB-sensor)

Source

This part is from Escherichia coli, Vibrio fischeri and Shewanella oneidensis,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015,BBa_R0062, BBa_K3102020 and BBa_K4767000.

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.

Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-considerations
+We fused the arsenic responding biosensor and the LuxR-based amplifier, using <i>mtrC</i> as a target gene to detect arsenic in the chassis of <i>Shewanella oneidensis</i> in a electroactive biofilm-based sensor (EAB-sensor)
 ===Source===
-Shewanella
+This part is from <i>Escherichia coli</i>, <i>Vibrio fischeri</i> and <i>Shewanella oneidensis</i>,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015,BBa_R0062, BBa_K3102020 and BBa_K4767000.
 ===References===
+Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.
+Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.