Difference between revisions of "Part:BBa K4882001"
 
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LqhIT2 is a 61 amino acid-long scorpion depressant toxin from L. quinquestriatus hebraeus. Although the scorpion is dangerous to humans, LqhIT2 is not. LqhIT2 shows a high preference for insect voltage-gated sodium channels and is totally harmless to mammals (Karbat et al., 2007; Zlotkin et al., 1991).
 
LqhIT2 is a 61 amino acid-long scorpion depressant toxin from L. quinquestriatus hebraeus. Although the scorpion is dangerous to humans, LqhIT2 is not. LqhIT2 shows a high preference for insect voltage-gated sodium channels and is totally harmless to mammals (Karbat et al., 2007; Zlotkin et al., 1991).
  
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===Usage and Biology===
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Since LqhIT2 is only toxic to insects, it is a good choice for pesticides. To improve the efficiency and effectiveness of Metarhizium anisopliae as a fungal biopesticide, we chose to introduce LqhIT2 into the fungus. LqhIT2 significantly improved the virulence of M. anisopliae against pests.
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<center><img src = "https://static.igem.wiki/teams/4882/wiki/part-lqhit2.png" style = "width:280px"></center>
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==References==
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Karbat, I.; Turkov, M.; Cohen, L.; Kahn, R.; Gordon, D.; Gurevitz, M.; Frolow, F. X-Ray Structure and Mutagenesis of the Scorpion Depressant Toxin LqhIT2
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<figcaption><center>Figure 1. The 3D structure of LqhIT2 (PDB ID: 2I61; Karbat et al., 2007). </center></figcaption>
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Zlotkin E, Eitan M, Bindokas VP, Adams ME, Moyer M, Burkhart W, Fowler E. Functional duality and structural uniqueness of depressant insect-selective neurotoxins. Biochemistry. 1991 May 14;30(19):4814-21. doi: 10.1021/bi00233a025. PMID: 2029523.
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<partinfo>BBa_K4882001 parameters</partinfo>
 
<partinfo>BBa_K4882001 parameters</partinfo>
 
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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Since LqhIT2 is only toxic to insects, it is a good choice for pesticides. To improve the efficiency and effectiveness of Metarhizium anisopliae as a fungal biopesticide, we chose to introduce LqhIT2 into the fungus. LqhIT2 significantly improved the virulence of M. anisopliae against pests.
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==References==
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Karbat, I.; Turkov, M.; Cohen, L.; Kahn, R.; Gordon, D.; Gurevitz, M.; Frolow, F. X-Ray Structure and Mutagenesis of the Scorpion Depressant Toxin LqhIT2
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Zlotkin E, Eitan M, Bindokas VP, Adams ME, Moyer M, Burkhart W, Fowler E. Functional duality and structural uniqueness of depressant insect-selective neurotoxins. Biochemistry. 1991 May 14;30(19):4814-21. doi: 10.1021/bi00233a025. PMID: 2029523.

Latest revision as of 14:45, 4 October 2023


LqhIT2, an insect-specific toxin from Leiurus quinquestriatus hebraeus

LqhIT2 is a 61 amino acid-long scorpion depressant toxin from L. quinquestriatus hebraeus. Although the scorpion is dangerous to humans, LqhIT2 is not. LqhIT2 shows a high preference for insect voltage-gated sodium channels and is totally harmless to mammals (Karbat et al., 2007; Zlotkin et al., 1991).

Figure 1. The 3D structure of LqhIT2 (PDB ID: 2I61; Karbat et al., 2007).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Since LqhIT2 is only toxic to insects, it is a good choice for pesticides. To improve the efficiency and effectiveness of Metarhizium anisopliae as a fungal biopesticide, we chose to introduce LqhIT2 into the fungus. LqhIT2 significantly improved the virulence of M. anisopliae against pests.

References

Karbat, I.; Turkov, M.; Cohen, L.; Kahn, R.; Gordon, D.; Gurevitz, M.; Frolow, F. X-Ray Structure and Mutagenesis of the Scorpion Depressant Toxin LqhIT2

Zlotkin E, Eitan M, Bindokas VP, Adams ME, Moyer M, Burkhart W, Fowler E. Functional duality and structural uniqueness of depressant insect-selective neurotoxins. Biochemistry. 1991 May 14;30(19):4814-21. doi: 10.1021/bi00233a025. PMID: 2029523.