Difference between revisions of "Part:BBa K4634007:Design"

Latest revision as of 05:15, 4 October 2023

pTargetF with gRNA designed

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 198
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 342
Illegal BamHI site found at 186
Illegal XhoI site found at 366
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1137
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 122

Design Notes

NA

Source

Lab stock

References

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. 10.1128/AEM.04023-14 PubMed 25636838

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	===References===		===References===
		+	Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. 10.1128/AEM.04023-14 PubMed 25636838