Difference between revisions of "Part:BBa K4897006"

 
(→‎Characterization)
 
(16 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4897006 short</partinfo>
 
<partinfo>BBa_K4897006 short</partinfo>
 +
===What is it?===
 +
Glutathione peroxidase7 (GPX 7) is an antioxidant enzyme in mammals in response to oxidative stress [1]. Oxidative stress essentially causes the aging of the skin and is typically caused by reactive oxygen species [2]. One of the reactive oxygen species that appear commonly on the human face is hydrogen peroxide. However, GPX7 can catalyze the reaction to reduce hydrogen peroxide to water and prevent the oxidation and aging of the skin [2],[3]. This is a way to nourish the skin in addition to the prevention of acne.
  
−
GPx7 (Glutathione peroxidase 7) is a non-selenocysteine active neutral antioxidant enzyme in mammals.
 
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
GPX7 is used for the nourishment of the skin, as it eliminates the presence of reacting oxygen species and hydrogen peroxide on the skin.
 +
 +
===Characterization===
 +
<html>
 +
<table width="100%" border="10" cellspacing="0" cellpadding="0">
 +
  <tr>
 +
    <td align="center"><img src="https://static.igem.wiki/teams/4897/wiki/parts/gpx7-purification.png" width="300" height="auto" /> </td>
 +
  </tr>
 +
  <tr>
 +
    <td align="center">Fig. 1. Characterization of GPx7 using Western Blotting
 +
</td>
 +
  </tr>
 +
</table>
 +
</html>
 +
Lane M is the marker, and Lane 1 is the purified GPX7 from E .coli BL21. The weight of GPX7 is around 21 kDa [4], and the graph shows that the purified His-GPX7 indeed has a weight of around 21 kDa.
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4897006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4897006 SequenceAndFeatures</partinfo>
  
 +
===References===
 +
[1]GPX7 glutathione peroxidase 7 [Homo sapiens (human)] - Gene - NCBI. (n.d.). Retrieved from www.ncbi.nlm.nih.gov website: https://www.ncbi.nlm.nih.gov/gene/2882
 +
<br>
 +
[2]Rinnerthaler, M., Bischof, J., Streubel, M., Trost, A., & Richter, K. (2015). Oxidative Stress in Aging Human Skin. Biomolecules, 5(2), 545–589. https://doi.org/10.3390/biom5020545
 +
<br>
 +
[3]UniProt. (n.d.). Retrieved October 4, 2023, from www.uniprot.org website: https://www.uniprot.org/uniprotkb/Q96SL4/entry
 +
<br>
 +
[4]Chen, Y.-I., Wei, P.-C., Hsu, J.-L., Su, F.-Y., & Lee, W.-H. (2016). NPGPx (GPx7): a novel oxidative stress sensor/transmitter with multiple roles in redox homeostasis. American Journal of Translational Research, 8(4), 1626–1640. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859894/
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 11:10, 8 October 2023


Antioxidant protein GPX7

What is it?

Glutathione peroxidase7 (GPX 7) is an antioxidant enzyme in mammals in response to oxidative stress [1]. Oxidative stress essentially causes the aging of the skin and is typically caused by reactive oxygen species [2]. One of the reactive oxygen species that appear commonly on the human face is hydrogen peroxide. However, GPX7 can catalyze the reaction to reduce hydrogen peroxide to water and prevent the oxidation and aging of the skin [2],[3]. This is a way to nourish the skin in addition to the prevention of acne.

Usage and Biology

GPX7 is used for the nourishment of the skin, as it eliminates the presence of reacting oxygen species and hydrogen peroxide on the skin.

Characterization

Fig. 1. Characterization of GPx7 using Western Blotting
Lane M is the marker, and Lane 1 is the purified GPX7 from E .coli BL21. The weight of GPX7 is around 21 kDa [4], and the graph shows that the purified His-GPX7 indeed has a weight of around 21 kDa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]GPX7 glutathione peroxidase 7 [Homo sapiens (human)] - Gene - NCBI. (n.d.). Retrieved from www.ncbi.nlm.nih.gov website: https://www.ncbi.nlm.nih.gov/gene/2882
[2]Rinnerthaler, M., Bischof, J., Streubel, M., Trost, A., & Richter, K. (2015). Oxidative Stress in Aging Human Skin. Biomolecules, 5(2), 545–589. https://doi.org/10.3390/biom5020545
[3]UniProt. (n.d.). Retrieved October 4, 2023, from www.uniprot.org website: https://www.uniprot.org/uniprotkb/Q96SL4/entry
[4]Chen, Y.-I., Wei, P.-C., Hsu, J.-L., Su, F.-Y., & Lee, W.-H. (2016). NPGPx (GPx7): a novel oxidative stress sensor/transmitter with multiple roles in redox homeostasis. American Journal of Translational Research, 8(4), 1626–1640. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859894/