Difference between revisions of "Part:BBa K4601051"
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The chloramphenicol ''O''-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic. | The chloramphenicol ''O''-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic. | ||
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Figure 1. The mode of action of the chloramphenicol ''O''-acetyltransferase enzyme: acetylation of chloramphenicol. | Figure 1. The mode of action of the chloramphenicol ''O''-acetyltransferase enzyme: acetylation of chloramphenicol. |
Latest revision as of 12:58, 3 October 2023
CmR
This part is CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (Uniprot P62577).
Usage and Biology
The chloramphenicol O-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic.
Figure 1. The mode of action of the chloramphenicol O-acetyltransferase enzyme: acetylation of chloramphenicol.
The CmR gene was isolated from the bacteriophage P1Cm carrying the Tncam-204 transposon derived from the R plasmid NR1 [1].
Reference
[1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]