Difference between revisions of "Part:BBa K228004"
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=='''Description'''== | =='''Description'''== | ||
− | + | We have constructed an inducement system composed of Psal promoter and its transcriptional actiator gene. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR. | |
It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression. | It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression. | ||
− | For the purpose of characterization, we connected [[Part:BBa_E0840|BBa_E0840]] downstream of the salicylate promoter to make the composite part, [[Part:BBa_K228850|BBa_K228850]]. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of [[Part:BBa_E0840|BBa_E0840]], while the input is salicylate solution at a gradient of concentration. | + | For the purpose of characterization, we connected [[Part:BBa_E0840|BBa_E0840]] downstream of the salicylate promoter to make the composite part, [[Part:BBa_K228850|BBa_K228850]]. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of [[Part:BBa_E0840|BBa_E0840]], while the input is salicylate solution at a gradient of concentration. |
=='''Performance'''== | =='''Performance'''== | ||
Line 27: | Line 27: | ||
|Maximum Output<br> | |Maximum Output<br> | ||
(Output tested in a time sequence) | (Output tested in a time sequence) | ||
− | |Click here | + | |[https://parts.igem.org/Part:BBa_K228004:Transfer#data2 Click here] |
|- | |- | ||
|Hill coefficient<br> | |Hill coefficient<br> | ||
(Output tested in a time sequence) | (Output tested in a time sequence) | ||
− | |Click here | + | |[https://parts.igem.org/Part:BBa_K228004:Transfer#data2 Click here] |
|- | |- | ||
− | |[[ |Switch Point]]<br> | + | |[[Part:BBa_K228004:Transfer|Switch Point]]<br> |
(Output tested in a time sequence) | (Output tested in a time sequence) | ||
− | |Click here | + | |[https://parts.igem.org/Part:BBa_K228004:Transfer#data2 Click here] |
|- | |- | ||
|Demand | |Demand | ||
− | |[[ |Decrease on Growth Rate]]<br> | + | |[[Part:BBa_K228004:Growth|Decrease on Growth Rate]]<br> |
(Input at a gradient of concentration) | (Input at a gradient of concentration) | ||
|The decrease level has significantly positive correlation with the input, the concentration of salicylate. | |The decrease level has significantly positive correlation with the input, the concentration of salicylate. | ||
Line 46: | Line 46: | ||
=='''Compatibility'''== | =='''Compatibility'''== | ||
− | '''Chassis''': Device has been shown to work in DH5α. | + | '''Chassis''': Device has been shown to work in DH5α.<br> |
− | '''Plasmids''': Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5 | + | '''Plasmids''': Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5<br> |
'''Devices''': Device has been shown to work with [[Part:BBa_K228013|BBa_K228013]], [[Part:BBa_K228227|BBa_K228227]] - [[Part:BBa_K228235|BBa_K228235]], [[Part:BBa_K228255|BBa_K228255]] - [[Part:BBa_K228263|BBa_K228263]], [[Part:BBa_K228851|BBa_K228851]], [[Part:BBa_K228852|BBa_K228852]] - [[Part:BBa_K228860|BBa_K228860]], [[Part:BBa_K228870|BBa_K228870]] - [[Part:BBa_K228878|BBa_K228878]]. | '''Devices''': Device has been shown to work with [[Part:BBa_K228013|BBa_K228013]], [[Part:BBa_K228227|BBa_K228227]] - [[Part:BBa_K228235|BBa_K228235]], [[Part:BBa_K228255|BBa_K228255]] - [[Part:BBa_K228263|BBa_K228263]], [[Part:BBa_K228851|BBa_K228851]], [[Part:BBa_K228852|BBa_K228852]] - [[Part:BBa_K228860|BBa_K228860]], [[Part:BBa_K228870|BBa_K228870]] - [[Part:BBa_K228878|BBa_K228878]]. | ||
+ | =='''Safety'''== | ||
+ | Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Latest revision as of 18:15, 21 October 2009
NahR( reverse) - salicylate promoter
Designed by Lin Min Group: iGEM09_PKU_Beijing (2009-09-18)
Input: Salicylate molecules
Output: GFP fluorescence
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]
Part Main Page | Transfer Function | Growth Rate |
Description
We have constructed an inducement system composed of Psal promoter and its transcriptional actiator gene. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.
It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.
For the purpose of characterization, we connected BBa_E0840 downstream of the salicylate promoter to make the composite part, BBa_K228850. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of BBa_E0840, while the input is salicylate solution at a gradient of concentration.
Performance
Experiment* | Characteristics* | Value & Result* |
Transfer Function | Maximum Output (Output tested in a time sequence) |
Click here |
Hill coefficient (Output tested in a time sequence) |
Click here | |
Switch Point (Output tested in a time sequence) |
Click here | |
Demand | Decrease on Growth Rate (Input at a gradient of concentration) |
The decrease level has significantly positive correlation with the input, the concentration of salicylate. |
Measured by Haoqian Zhang & Chengzhe Tian, 2009
Compatibility
Chassis: Device has been shown to work in DH5α.
Plasmids: Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5
Devices: Device has been shown to work with BBa_K228013, BBa_K228227 - BBa_K228235, BBa_K228255 - BBa_K228263, BBa_K228851, BBa_K228852 - BBa_K228860, BBa_K228870 - BBa_K228878.
Safety
Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.