Difference between revisions of "Part:BBa K228004"
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''Input'': Salicylate molecules<br>
 
''Input'': Salicylate molecules<br>
 
''Output'': GFP fluorescence<br>
 
''Output'': GFP fluorescence<br>
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<partinfo>BBa_K228004 SequenceAndFeatures</partinfo>
  
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{|cellpadding=3
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{|cellpadding=5 style="background:gray"
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|  Part Main Page  ||  Transfer Function  ||  Growth Rate   
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|[[Part:BBa_K228004|  Part Main Page  ]]||[[Part:BBa_K228004:Transfer|  Transfer Function  ]]||[[Part:BBa_K228004:Growth|  Growth Rate  ]]
 
|}
 
|}
  
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=='''Description''===
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=='''Description'''==
  
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This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.  
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We have constructed an inducement system composed of Psal promoter and its transcriptional actiator gene. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.  
  
 
It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.  
 
It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.  
  
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For the purpose of characterization, we connected BBa_E0840 downstream of the salicylate promoter to make the composite part, BBa_K228850. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of BBa_E0840, while the input is salicylate solution at a gradient of concentration.  
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For the purpose of characterization, we connected [[Part:BBa_E0840|BBa_E0840]] downstream of the salicylate promoter to make the composite part, [[Part:BBa_K228850|BBa_K228850]]. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of [[Part:BBa_E0840|BBa_E0840]], while the input is salicylate solution at a gradient of concentration.
  
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=='''Performance'''==
  
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{|cellpadding=3
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|Experiment*||Characteristics*||Value & Result*
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|-
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|rowspan=3|[[Part:BBa_K228004:Transfer|Transfer Function]]
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|Maximum Output<br>
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(Output tested in a time sequence)
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|[https://parts.igem.org/Part:BBa_K228004:Transfer#data2 Click here]
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|-
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|Hill coefficient<br>
 +
(Output tested in a time sequence)
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|[https://parts.igem.org/Part:BBa_K228004:Transfer#data2 Click here]
 +
|-
 +
|[[Part:BBa_K228004:Transfer|Switch Point]]<br>
 +
(Output tested in a time sequence)
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|[https://parts.igem.org/Part:BBa_K228004:Transfer#data2 Click here]
 +
|-
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|Demand
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|[[Part:BBa_K228004:Growth|Decrease on Growth Rate]]<br>
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(Input at a gradient of concentration)
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|The decrease level has significantly positive correlation with the input, the concentration of salicylate.
 +
|}
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Measured by Haoqian Zhang & Chengzhe Tian, 2009
  
 +
=='''Compatibility'''==
  
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[[Image:PKU_Salicylate.jpg|600px]]
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'''Chassis''': Device has been shown to work in DH5α.<br>
 +
'''Plasmids''': Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5<br>
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'''Devices''': Device has been shown to work with [[Part:BBa_K228013|BBa_K228013]], [[Part:BBa_K228227|BBa_K228227]] - [[Part:BBa_K228235|BBa_K228235]], [[Part:BBa_K228255|BBa_K228255]] - [[Part:BBa_K228263|BBa_K228263]], [[Part:BBa_K228851|BBa_K228851]], [[Part:BBa_K228852|BBa_K228852]] - [[Part:BBa_K228860|BBa_K228860]], [[Part:BBa_K228870|BBa_K228870]] - [[Part:BBa_K228878|BBa_K228878]].
  
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=='''Safety'''==
  
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<!-- Add more about the biology of this part here
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Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K228004 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 18:15, 21 October 2009

NahR( reverse) - salicylate promoter

Designed by Lin Min Group: iGEM09_PKU_Beijing (2009-09-18)
Input: Salicylate molecules
Output: GFP fluorescence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]
Part Main Page Transfer Function Growth Rate

Description

We have constructed an inducement system composed of Psal promoter and its transcriptional actiator gene. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.

It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.

For the purpose of characterization, we connected BBa_E0840 downstream of the salicylate promoter to make the composite part, BBa_K228850. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of BBa_E0840, while the input is salicylate solution at a gradient of concentration.

Performance

Experiment* Characteristics* Value & Result*
Transfer Function Maximum Output

(Output tested in a time sequence)

Click here
Hill coefficient

(Output tested in a time sequence)

Click here
Switch Point

(Output tested in a time sequence)

Click here
Demand Decrease on Growth Rate

(Input at a gradient of concentration)

The decrease level has significantly positive correlation with the input, the concentration of salicylate.

Measured by Haoqian Zhang & Chengzhe Tian, 2009

Compatibility

Chassis: Device has been shown to work in DH5α.
Plasmids: Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5
Devices: Device has been shown to work with BBa_K228013, BBa_K228227 - BBa_K228235, BBa_K228255 - BBa_K228263, BBa_K228851, BBa_K228852 - BBa_K228860, BBa_K228870 - BBa_K228878.

Safety

Because this part is from commonly used elements in bacteria. No safety problem can be arised by this part.