Difference between revisions of "Part:BBa K4839013"

 
 
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<partinfo>BBa_K4839013 short</partinfo>
 
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The mecherry used in this study is coden optimized in THP-1 cell for neagative control
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The mecherry used in this study is coden optimized in THP-1 cell for neagative control.(Figure1)
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<html><img src="https://static.igem.wiki/teams/4839/wiki/parts-files/9-1.png" width="650"</html>
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<p align="center">Figure1. The prove-of-concept of our SNIPR design</p>
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<p>[1] Zhu I , Liu R , Wittsten A H ,et al.Design and modular assembly of synthetic intramembrane proteolysis receptors for custom gene regulation in therapeutic cells[J]. 2021.DOI:10.1101/2021.05.21.445218.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:31, 3 October 2023


mCherry

The mecherry used in this study is coden optimized in THP-1 cell for neagative control.(Figure1)


Figure1. The prove-of-concept of our SNIPR design

[1] Zhu I , Liu R , Wittsten A H ,et al.Design and modular assembly of synthetic intramembrane proteolysis receptors for custom gene regulation in therapeutic cells[J]. 2021.DOI:10.1101/2021.05.21.445218.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]